منابع مشابه
Computer program for calculating the melting temperature of degenerate oligonucleotides used in PCR or hybridization.
Degenerate primers or probes have been widely used in molecular cloning, but the calculation of their melting temperatures could not simply be done using thermodynamic parameters because of degeneracy and the lack of a computer program. We present here a simple computer program named dPrimer for the calculation of melting temperature of degenerate oligonucleotides based on the nearest-neighbor ...
متن کاملDetermination of melting temperature and temperature melting range for DNA with multi-peak differential melting curves.
Many factors that change the temperature position and interval of the DNA helix-coil transition often also alter the shape of multi-peak differential melting curves (DMCs). For DNAs with a multi-peak DMC, there is no agreement on the most useful definition for the melting temperature, Tm, and temperature melting width, ΔT, of the entire DNA transition. Changes in Tm and ΔT can reflect unstable ...
متن کاملMELTING, computing the melting temperature of nucleic acid duplex
UNLABELLED MELTING computes the enthalpy and entropy of an oligonucleotide duplex helix-coil transition, and then its melting temperature. The program uses the method of nearest-neighbours. The set of thermodynamic parameters can be easily customized. The program provides several correction methods for the concentration of salt. MELTING is a free program, available at no cost and open-source. P...
متن کاملInfluence of Size on the Melting Temperature of Metallic Nanoparticle
In This paper, the effect of size on melting temperature of metallic nanoparticles (Au, Pb and Bi) is theoretically simulated and explained. In this regard, the cause of difference in various experimental data is introduced, which is the difference between nanoparticles’ grain Gaussian distribution. This volume-depended model with the help of the Gaussian distribution can descri...
متن کاملInvestigating the highest melting temperature materials: A laser melting study of the TaC-HfC system
TaC, HfC and their solid solutions are promising candidate materials for thermal protection structures in hypersonic vehicles because of their very high melting temperatures (>4000 K) among other properties. The melting temperatures of slightly hypostoichiometric TaC, HfC and three solid solution compositions (Ta1-xHfxC, with x = 0.8, 0.5 and 0.2) have long been identified as the highest known....
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ژورنال
عنوان ژورنال: Nature
سال: 1986
ISSN: 0028-0836,1476-4687
DOI: 10.1038/323485a0